Course unit code:
N423U0_4B
Course unit title:
Introduction to Bioprocesses
Mode of delivery, planned learning activities and teaching methods:
lecture – 2 hours weekly (on-site method)
laboratory practice – 2 hours weekly (on-site method)
Credits allocated:
4
Recommended semester:
Chemical Engineering – bachelor (full-time, attendance method), 4. semester
Automation, Information Engineering and Management in Chemistry and Food Industry – bachelor (full-time, attendance method), 4. semester
Level of study:
1.
Prerequisites for registration:
none
Assesment methods:
Students write two written tests from problems for 50 points in total. To pass them, minimum 27.5 points are needed. A written exam from the theory is classified by 50 points. A minimum needed is 27.5 points. A student will receive credits if both parts are passed successfully. Exam grades are as follows: A 91-100 points, B 82-90 points, C 73-81 point, D 64-72 points, E 55-63 points.
Learning outcomes of the course unit:
Students obtain knowledge about the cell structure and properties in relation to their performance in the environment of biotechnological processes and about basic stoichiometric calculations and determination of kinetic parameters.
Course contents:
1. Microbiology
cell structure, eukaryots, prokaryots
groups of microorganisms – bacteria, yeasts, mycellial fungi, algae, animal and plant cells
aerobes and anaerobes
cell nutrition, composition of cultivation media, life cycle

2. Biochemistry
basic biochemical compounds – lipids, saccharides, DNA and RNA, amino acids and proteins. Structure and function in living organisms.

3. Principle biotechnological production processes
Brief of overview of biotechnological production processes. Amino acids. Enzymes. Antibiotics. Ethanol. Organic acids. Polysaccharides. Vitamins. Specialty chemicals.

4. Metabolic stoichiometry and energetics
Principles of production and consumption of energy inside the cells (ADP and ATP), form of carbon catabolism, respiration chain, biosynthesis of small molecules and macromolecules, cell membrane transport and principle regulatory mechanisms. Application of this knowledge on the production processes of final metabolites
Stechiometry of cell growth and product formation, yield coefficients
Thermodynamics of cell cultivations

5. Laboratory exercises
Determination of enzyme kinetics using the initial rate method
Determination of kinetic parameters of Michaelis-Menten equation using the progress curve method
Recommended or required reading:
Basic:
  • ŠKÁRKA, B. Biochémia. Bratislava: Alfa, 1999.
  • HUDECOVÁ, D. – MAJTÁN, V. Mikrobiológia I. Bratislava: Vydavateľstvo STU v Bratislave, 2002. 189 p. ISBN 80-227-1663-4.
  • BÁLEŠ, V. – MÉSZÁROS, A. – POLAKOVIČ, M. – ŠTEFUCA, V. – MUNTEAN, O. Biochemické technológie: Biochemical Technologies. Bratislava : AB-ART, 2003. 128 p. ISBN 80-89006-75-2.
  • DORAN, P M. Bioprocess Engineering Principles. London : Academic Press, 1995. 439 p. ISBN 0-12-220856-0.
Language of instruction:
Slovak, English
Notes:
The course is compulsory for the students of the study program Chemical Engineering
Assessed students in total:
206

A 5.3 %

B 13.6 %

C 20.9 %

D 34 %

E 20.4 %

FX 5.8 %

Course supervisor:
prof. Ing. Milan Polakovič, CSc.
Last modification:
16. 1. 2018

Department:
Department of Chemical and Biochemical Engineering

AIS: 2017/2018  

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